1 Describe the theory and application of nucleic acid extraction.
2 Demonstrate the ability to prepare nucleic specimens for analysis and assess its quality including the identification of sources of interference/errors.
3* Measure the absorbance/fluorescence of nucleic acids to identify the suitability and concentration of nucleic acid extracts for molecular testing using spectrophotometers and fluorometers.
4 Perform QC and QA in the molecular biology laboratory.
5 Describe the theory and application of cloning vectors.
6 Describe the theory and application of restriction enzyme digestion.
7 Describe the historical, present and anticipated future of molecular techniques and complimentary technologies, including nucleic acid amplification, hybridization and sequencing methodologies, in research and as a diagnostic tool.
8 Demonstrate the ability to design primers to a specific nucleic acid segment for detection and amplification
9 Perform PCR procedures to detect genetic variations, including the assessment of results.
10 Describe the theory and application of Northern, Southern and Western blot electrophoresis and techniques and primer/probe labelling.
11 Perform electrophoresis including the capturing of results as an image using gel documentation software for assessment.
12 Apply professional practice according to established protocols, safety guidelines, and existing legislation
13 Operate standard laboratory equipment/instruments
14 Follow established protocols as defined in policy, process and procedure manuals
15 Apply effective communication and teamwork skills through completion of laboratory activities that require collaboration and cooperation
16 Apply critical thinking skills to identify, evaluate and problem solve sources of error associated with molecular biology analysis including the initiation of corrective action
17 Demonstrate professionalism through compliance with the expectation to produce individual results, taking responsibility for actions, recognizing limitations, and being accountable for professional actions including scope of practice, accountability and professional development.
18 Recognizes relationships between analyses, diagnoses, clinical information and treatment by assessing molecular based assay results based on specimen integrity, reference values, critical values, method limitations, clinical conditions and other laboratory findings.
19* Prepare bodily fluids for FISH analysis by separating, culturing, and
synchronizing cells.
20* Interpret test results from in situ techniques by detecting and localizing specific nucleic acid sequences in cells, tissue sections and whole tissue.
* = This is an essentially new learning outcome to align with CAMPLR as opposed to a reorganization of existing LOs
